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Indirect haemagglutination test using monoclonal antibody-affinity purified antigens for diagnosis of human paragonimiasis due to Paragonimus heterotremus

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Abstract:

An indirect haemagglutination test (IHA) using antigens purified by monoclonal antibody-affinity chromatography was developed for the diagnosis of human paragonimiasis caused by Paragonimus heterotremus. Sera from patients with paragonimiasis (n= 30) were evaluated, along with sera from other parasitic infections (n= 92), pulmonary tuberculosis (n= 18) and healthy controls (n = 30). The sensitivity, specificity as well as positive and negative predictive value of the IHA, calculated at the prevalence of disease at 17.6%, were all 100%.

Keywords: Paragonimus heterotremus; affinity purified antigens; diagnosis; indirect haemagglutination test; paragonimiasis

Document Type: Research Article

DOI: http://dx.doi.org/10.1046/j.1365-3156.1998.00173.x

Affiliations: 1: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Thailand, 2: Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Thailand, 3: Institute of Medicine, Suranaree University of Technology, Nakhonratchasima, Thailand, 4: Department of Parasitology, Faculty of Medicine, Chiang Mai University, Thailand, 5: Department of Microbiology and Immunology, Faculty of Tropical Medicine, Bangkok, Thailand

Publication date: January 1, 1998

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