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pOVEX vector: prokaryotic expression and purification of onchocerciasis vaccine candidate antigens as fusion proteins with the 24 kD Onchocerca volvulus glutathione S-transferase

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Abstract:

An expression vector, pOVEX, has been designed and constructed, combining the advantages of the expression vectors pGEX-3X and pJC20. The pOVEX vector produces a fusion protein with the 24 kD Onchocerca volvulus glutathione S-transferase (OvGST2) which is easy to purify in one step from bacterial extracts under non-denaturing conditions using glutathione-sepharose chromatography. High yields of fusion protein were produced from this T7 RNA polymerase-dependent expression vector, which were then cleaved by digestion with the factor Xa protease to separate the OVGST2 polypeptide from the expressed protein of interest. This vector will be particularly useful to O. volvulus investigators for the production of O. volvulus antigens for the analyses of host humoural and cellular responses to these proteins and for immunization studies.

Keywords: Onchocerca volvulus; S-transferase; expression vector; glutathione; recombinant proteins

Document Type: Original Article

DOI: http://dx.doi.org/10.1046/j.1365-3156.1997.d01-352.x

Affiliations: Department of Biochemistry, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany

Publication date: July 1, 1997

bsc/tmih/1997/00000002/00000007/art00012
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