Actin polymerization stimulated by contractile activation regulates force development in canine tracheal smooth muscle
The role of actin polymerization in the regulation of smooth muscle contractility was investigated in canine trachealis muscle strips. The effect of contractile activation on the content of monomeric globular (G)‐actin was estimated by the method of DNase I inhibition. The G‐actin content was 30 % lower in extracts of muscle strips activated with 10−4 M acetylcholine (ACh) than in extracts from unstimulated muscle strips. The decrease in G‐actin in response to contractile stimulation was prevented by latrunculin‐A, an agent that prevents actin polymerization by binding to G‐actin monomers.
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Document Type: Research Article
Affiliations: Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis, IN 46202, USA
Publication date: 01 September 1999