Authors: Masunaga, Yoshinori; Muto, Shigeaki; Asakura, Shinji; Akimoto, Tetsu; Homma, Sumiko; Kusano, Eiji; Asano, Yasushi

Source: Therapeutic Apheresis and Dialysis, Volume 7, Number 5, October 2003 , pp. 486-493(8)

Publisher: Wiley-Blackwell

Abstract:

: 

Encapsulating peritoneal sclerosis (EPS) remains one of the major causes of dropout in continuous ambulatory peritoneal dialysis by reducing ultrafiltration capacity. To demonstrate whether ascites from patients with EPS (EPS ascites) has fibroblast proliferation activity, we used NIH/3T3 fibroblasts to examine the effects of EPS ascites on fibroblast proliferation activity by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Encapsulating peritoneal sclerosis ascites dose-dependently augmented NIH/3T3 fibroblast proliferation. The protein kinase C inhibitors and the tyrosine kinase inhibitors partially inhibited the stimulatory effects of EPS ascites on fibroblast proliferation activity. In EPS ascites, levels of interleukin (IL)-1β, IL-6, IL-8, transforming growth factor (TGF)-β1, hepatocyte growth factor (HGF), and platelet-derived growth factor (PDGF)-AB were elevated. The treatment with IL-1β, HGF, TGF-β1, and PDGF-AB alone or in combination at similar concentrations to those in EPS ascites exhibited small but significant fibroblast proliferation activities. We conclude that EPS ascites stimulate NIH/3T3 fibroblast proliferation via protein kinase C and tyrosine kinase. The elevated cytokine and growth factors partly contribute to the EPS ascites-induced fibroblast proliferation.

Keywords: Continuous ambulatory peritoneal dialysis; Cytokines; Growth factors; MTT assay; Protein kinase C; Tyrosine kinase

Document Type: Research article

DOI: http://dx.doi.org/10.1046/j.1526-0968.2003.00087.x

Affiliations: 1: Department of Nephrology, Jichi Medical School, Minamikawachi, Kawachi, Tochigi, Japan

Publication date: 2003-10-01

Related content

• In this: publication
• By this: publisher
• In this Subject: Therapeutics & Alternative Medicine
• By this author: Masunaga, Yoshinori ;  Muto, Shigeaki ;  Asakura, Shinji ;  Akimoto, Tetsu ;  Homma, Sumiko ;  Kusano, Eiji ;  Asano, Yasushi

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers