Authors: Oestergaard, S.¹; Rasmussen, K. E.¹; Doyle, N.²; Varela, A.²; Chouinard, L.²; Smith, S. Y.¹; Qvist, P.¹; Karsdal, M. A.¹

Source: Scandinavian Journal of Immunology, Volume 67, Number 3, March 2008 , pp. 304-312(9)

Publisher: Wiley-Blackwell

Abstract:

The purpose of this work was to validate collagen antibody-induced arthritis (CAIA) model in two mice strains (Balb/c and CD-1) using clinical, biochemical, microstructural and histological techniques. We induced arthritis in mice using a cocktail of collagen type II (CII) antibodies followed by an injection with lipopolysaccharide (LPS) in different doses in Balb/c and CD-1 mice strains. Serum CTX-II levels were measured at study termination and correlated with microscopic severity of joint lesions as determined by a validated scoring systems. Bone involvement was assessed by microcomputer tomography (micro-CT). Balb/c mice developed rapid (day 6) and robust (100%) arthritis, whereas CD-1 mice showed only temporary macroscopic signs of disease. Serum CTX-II levels in Balb/c mice showed a significant increase in cartilage degradation in diseased animals (43-64% compared with non-diseased mice) and was decreased in animals receiving dexamethasone. Correlation of serum CTX-II with the microscopic score was statistically significant (P < 0.01). Micro-CT analysis demonstrated structural damage in bone in the CAIA Balb/c mice, which was prevented by dexamethasone. The CAIA-LPS model provides a useful supplement to currently available animal models of arthritis. This is a rapid onset and robust model; however, the choice of mouse strain should be evaluated carefully.

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1365-3083.2007.02060.x

Affiliations: 1: Nordic Bioscience A/S, Herlev, Denmark 2: Charles River Laboratories, Preclinical Services Montreal, Transcanadienne, Senneville, QC, Canada

Publication date: 2008-03-01

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• In this Subject: Microbiology ,  Allergy & Immunology
• By this author: Oestergaard, S. ;  Rasmussen, K. E. ;  Doyle, N. ;  Varela, A. ;  Chouinard, L. ;  Smith, S. Y. ;  Qvist, P. ;  Karsdal, M. A.

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