Protective effect of a multiantigenic DNA vaccine against Toxoplasma gondii with co-delivery of IL-12 in mice

Authors: CUI, Y.-L.; HE, S.-Y.; XUE, M.-F.; ZHANG, J.; WANG, H.-X.; YAO, Y.

Source: Parasite Immunology, Volume 30, Number 5, May 2008 , pp. 309-313(5)

Buy & download fulltext article:

You have access to the full text article on a website external to ingentaconnect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library

Abstract:

SUMMARY

In this study, we constructed a multiantigenic DNA vaccine, pSAG1-ROP2-SAG2 and examined its effect with co-delivery of a plasmid encoding IL-12 (pIL-12) as an adjuvant in BALB/c mice against Toxoplasma gondii. After a lethal challenge of T. gondii RH strain, survival of the mice immunized with this pSAG1-ROP2-SAG2 vaccine was significantly prolonged in comparison to the control groups. Furthermore, the protection was significantly augmented by pIL-12 co-delivery. As demonstrated by lymphocyte proliferation assay, cytokine and antibody level determinations, the humoral and Th1-type cellular responses elicited by this multiantigenic DNA vaccine were significantly stronger than those elicited by double-antigenic, or single-antigenic DNA vaccines. Our data suggest that multiantigenic DNA vaccine with pIL-12 co-delivery is a very effective approach in the protection against T. gondii.

Keywords: DNA vaccine; IL-12; multiantigenic ROP2; SAG1; SAG2; Toxoplasma gondii

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1365-3024.2008.01025.x

Publication date: 2008-05-01