38-48 kDa subunits of buckwheat 13S globulins are controlled by a single locus

Authors: Luthar, Z.¹; Rogl, S.²; Kump, B.¹; Javornik, B.

Source: Plant Breeding, Volume 127, Number 3, June 2008 , pp. 322-324(3)

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Abstract:

The inheritance of buckwheat (Fagopyrum esculentum Moench) seed storage proteins was investigated by control crosses between accessions containing different SDS-PAGE protein patterns in the range of 38-48 kDa. Analysis of segregation of individual F₁ and F₂ seeds obtained by 17 crosses showed segregation of eight electrophoretic bands, from which 12-banding variants were inferred. Two variants contained one band, nine contained two bands and one variant contained three bands and behaved as a single co-dominant unit. The segregation of banding variants fits the expected ratios and thus supports the hypothesis of single Mendelian gene inheritance and that alleles are co-dominant. The results therefore suggest that the genes controlling globulin subunits are tightly linked and inherited as a single locus. The locus was designated Glob-1 and the twelve segregated protein variants as alleles a-l. This is the first buckwheat globulin locus identified with multiple alleles, phenotypically expressed as groups of protein bands and thus applicable as a functional marker in buckwheat genomic studies.

Keywords: seed storage proteins; SDS-PAGE; inheritance; Fagopyrum esculentum Moench

Document Type: Short communication

DOI: http://dx.doi.org/10.1111/j.1439-0523.2007.01466.x

Affiliations: 1: Agronomy Department, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia 2: Ministry of Agriculture, Food and Forestry, Dunajska c. 52, 1000 Ljubljana, Slovenia

Publication date: 2008-06-01