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Genetic mapping of the barley Rrs14 scald resistance gene with RFLP, isozyme and seed storage protein markers

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The scald susceptible barley cultivar ‘Clipper’ and a third-backcross (BC3) line homozygous for the Rrs14 scald resistance gene that originally came from Hordeum vulgare ssp. spontaneum were grown in replicated field trials. The level of resistance that Rrs14 confers against field populations of the pathogen Rhynchosporium secalis, the causal agent of scald disease, was evaluated. The Rrs14 BC3 line exhibited 80% and 88% less leaf damage than ‘Clipper’ in 1995 and 1996, respectively. Given this effectiveness of Rrs14, research was undertaken to identify a linked marker locus suitable for indirect selection of Rrs14. Based on linkage to a set of previously mapped loci, Rrs14 was positioned to barley chromosome 1H between the seed storage protein (hordein) loci Hor1 and Hor2, approximately 1.8cM from the latter locus. The Hor2 locus is thus an ideal codominant molecular marker for Rrs14. The tight linkage between Rrs14 and Hor2 and the availability of alternative biochemical and molecular techniques for scoring Hor2 genotypes, permits simple indirect selection of Rrs14 in barley scald resistance breeding programmes.
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Keywords: Hordeum vulgare ssp. spontaneum; Rhynchosporium secalis; hordeins; molecular markers; pyramiding resistance genes; scald disease

Document Type: Research Article

Affiliations: 1: CRC for Plant Science, GPO Box 475, Canberra, ACT 2601, Australia; 2: Centre for Plant Biodiversity Research, CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia; 3: NSW Agriculture, Agricultural Institute, PMB, Wagga Wagga NSW 2650, Australia;

Publication date: 01 January 2000

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