Free Content Engulfment-regulated proteolysis of SpoIIQ: evidence that dual checkpoints control K activity

You have access to the full text article on a website external to ingentaconnect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library

Download Article:

Abstract:

Summary

During Bacillus subtilis sporulation, the engulfment checkpoint is thought to directly regulate late forespore transcription but to indirectly regulate late mother cell transcription, via the G-produced protease SpoIVB. We here demonstrate that SpoIIQ is subject to G-independent, but engulfment-dependent, proteolysis that depends on SpoIVB. Thus, SpoIVB produced before engulfment supports some SpoIVB-dependent events, suggesting that its activity or access to substrates must be regulated by engulfment. Furthermore, a mutation (bofA) that allows K to be active without G does not allow K activity in engulfment mutants, although the pro-K processing enzyme (SpoIVFB) is localized to the septum in engulfment mutants, suggesting that engulfment comprises a second checkpoint for K Finally, we find that SpoIIQ and another protein required for G activity (SpoIIIAH), which directly interact and assemble helical structures around the forespore, recruit the K-processing enzyme SpoIVFB to the forespore and these structures. We suggest that these foci serve a synapse-like role, allowing engulfment to simultaneously control both G and K, and integrating multiple checkpoints and signalling pathways.

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1365-2958.2005.04811.x

Affiliations: Division of Biological Sciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0377, USA.

Publication date: October 1, 2005

Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more