Authors: Morgan, Eirwen¹; Campbell, June D.¹; Rowe, Sonya C.¹; Bispham, Jennie¹; Stevens, Mark P.¹; Bowen, Alison J.¹; Barrow, Paul A.¹; Maskell, Duncan J.²; Wallis, Timothy S.¹

Source: Molecular Microbiology, Volume 54, Number 4, November 2004 , pp. 994-1010(17)

Publisher: Wiley-Blackwell

Abstract:

Summary

The severity of infections caused by Salmonella enterica serovar Typhimurium varies depending on the host species. Numerous virulence genes have been identified in S. Typhimurium, largely from studies in mice, but their roles in infections of other species remain unclear. In the most comprehensive survey of its kind, through the use of signature-tagged mutagenesis of S. Typhimurium we have identified mutants that were unable to colonize calf intestines, mutants unable to colonize chick intestines and mutants unable to colonize both species. The type three secretion systems encoded on Salmonella pathogenicity islands (SPIs) 1 and 2 were required for efficient colonization of cattle. However, disruption of these secretion systems only caused a minor defect in S. Typhimurium colonization of chicks. Transposon insertions in SPI-4 compromised S. Typhimurium colonization of cattle, but not chicks. This is the first data confirming a role for SPI-4 in pathogenesis. We have also been able to ascribe a role in colonization for cell surface polysaccharides, cell envelope proteins, and many `housekeeping' genes and genes of unknown function. We conclude that S. Typhimurium uses different strategies to colonize calves and chicks. This has major implications for vaccine design.

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1365-2958.2004.04323.x

Affiliations: 1: Division of Microbiology, Institute for Animal Health, Compton, Nr Newbury, RG20 7NN, UK. 2: Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, CB3 0ES UK.

Publication date: 2004-11-01

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• By this author: Morgan, Eirwen ;  Campbell, June D. ;  Rowe, Sonya C. ;  Bispham, Jennie ;  Stevens, Mark P. ;  Bowen, Alison J. ;  Barrow, Paul A. ;  Maskell, Duncan J. ;  Wallis, Timothy S.

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