PCR-RFLP markers detect 29 mitochondrial haplotypes in Pacific lamprey (Entosphenus tridentatus)

Authors: DOCKER, M. F.¹; HAAS, G. R.²; GOODMAN, D. H.³; REID, S. B.⁴; HEATH, D. D.¹

Source: Molecular Ecology Notes, Volume 7, Number 2, March 2007 , pp. 350-353(4)

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Abstract:

We developed five polymerase chain reaction-based markers that detect variation in the mitochondrial genome of the Pacific lamprey, Entosphenus tridentatus, across most of its range. Two gene fragments (ND2 and ND5) were amplified and digested with three and two restriction enzymes, respectively, detecting sequence variation at 18 sites (ND2 = 13; ND5 = 5) and yielding 29 composite haplotypes among 1246 lampreys. These sequence-based markers will be useful in a range of phylogeographical and population genetic studies.

Keywords: Entosphenus tridentatus; genetic variation; Lampetra tridentata; mitochondrial DNA; Pacific lamprey; PCR-RFLP

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1471-8286.2006.01605.x

Affiliations: 1: Great Lakes Institute for Environmental Research, University of Windsor, Windsor, Ontario, Canada N9B 3P4, 2: Institute of Marine Science, University of Alaska Fairbanks, Fairbanks, AK 99775-7220, USA, 3: Department of Fisheries Biology, Humboldt State University, Arcata, CA 95521, USA, 4: Western Fishes, 2045 East Main St, Ashland, OR 97520, USA

Publication date: 2007-03-01