Isolation and characterization of microsatellite markers from Pacific white shrimp (Litopenaeus vannamei)

Authors: ZHI-YING, JIA; XIAO-WEN, SUN¹; LI-QUN, LIANG¹; DA-YU, LI²; QING-QUAN, LEI³

Source: Molecular Ecology Notes, Volume 6, Number 4, December 2006 , pp. 1282-1284(3)

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Abstract:

We report the development of 11 polymorphic microsatellite loci in pacific white shrimp (Litopenaeus vannamei) using an unenriched genomic library. The number of the alleles ranged from two to 18 and observed hererozygosity ranged from 0.0286 to 0.9429, indicating that these markers will be useful for population studies and mapping in pacific white shrimp. Seven loci were detected deviated from Hardy-Weinberg, caused by deficiency of heterozygote, suggesting population genetic structure across the sampled population. No evidence for linkage disequilibrium was found.

Keywords: Litopenaeus vannamei; microsatellites; primers; white shrimp

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1471-8286.2006.01515.x

Affiliations: 1: Key Laboratory of Bioengineering Breeding of Northern Fishing, Ministry of Agriculture, China, Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China, 2: College of Aqua-life Science and Technology, Dalian Fisheries University, 116023, China, 3: Material Science and Engineering College, Harbin Science and Technology University, Harbin 150080, China

Publication date: 2006-12-01