Characterization of 59 canine single nucleotide polymorphisms in the Italian wolf (Canis lupus) population

Authors: ANDERSEN, DITTE HOLM¹; FABBRI, ELENA¹; SANTINI, ALBERTO¹; PAGET, SANDRINE²; CADIEU, EDOUARD²; GALIBERT, FRANCIS²; ANDRÉ, CATHERINE²; RANDI, ETTORE¹

Source: Molecular Ecology Notes, Volume 6, Number 4, December 2006 , pp. 1184-1187(4)

Publisher: Wiley-Blackwell

Buy & download fulltext article:

Price: $48.00 plus tax (Refund Policy)

Abstract:

We characterized 59 canine single nucleotide polymorphisms (SNPs) in the endangered Italian wolf (Canis lupus) population, which were discovered by resequencing sequence-tagged-site (STS) DNA sequences that are known to contain SNPs in domestic dogs. Dog SNPs were usually found also in wolves. Additional SNPs unique in dogs or wolves were discovered, which is important for detecting hybrids between dogs and wolves. We developed new primer sets and analysed 15 SNPs by Pyrosequencing. The characterized SNPs will provide an important addition to the genetic markers that are currently available for studying wild populations of canids.

Keywords: Canis lupus; Italian wolf; single nucleotide polymorphism

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1471-8286.2006.01486.x

Affiliations: 1: Istituto Nazionale per la Fauna Selvatica, Via Cà Fornacetta 9, 40064 Ozzano dell'Emilia (BO), Italy, 2: UMR 6061 CNRS, Génétique et Développement, Faculté de Médecine, Université de Rennes1, 35043 RENNES Cédex, France

Publication date: 2006-12-01