Ten microsatellite loci for evolutionary research on Nesospiza buntings
Authors: DELPORT, WAYNE; GRANT, TYRON J.1; RYAN, PETER G.2; BLOOMER, PAULETTE
Source: Molecular Ecology Notes, Volume 6, Number 4, December 2006 , pp. 1180-1183(4)
Publisher: Blackwell Publishing
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Abstract:
We tested 19 loci originally developed for Vidua and Geospiza, of which six Vidua loci were polymorphic and furthermore, developed four microsatellite loci for Nesospiza buntings. Allelic variation ranged from two to 13 alleles, with heterozygosity from 0 to 0.82. Ascertainment bias was evident as increased allelic diversity of loci developed for Nesospiza, versus those developed for Vidua. Two loci showed linkage, and several, deviation from Hardy-Weinberg equilibrium. We suggest such deviations are through introduction of new alleles by introgression, or of the Wahlund effect. These loci will contribute to the understanding of speciation in Nesospiza buntings of the Tristan da Cunha islands.Keywords: FIASCO; microsatellites; Nesospiza; speciation; Tristan da Cunha islands
Document Type: Research article
DOI: 10.1111/j.1471-8286.2006.01485.x
Affiliations: 1: Molecular Ecology and Evolution Programme, Department of Genetics, University of Pretoria, Pretoria 0002, South Africa, 2: DST-NRF Centre of Excellence at the Percy FitzPatrick Institute, University of Cape Town, Rondebosch, Cape Town 7701, South Africa
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