Development of SSR markers located in the G1 linkage group of apricot (Prunus armeniaca L.) using a bacterial artificial chromosome library

Authors: VILANOVA, S.; SORIANO, J. M.¹; LALLI, D. A.²; ROMERO, C.¹; ABBOTT, A. G.²; LLÁCER, G.¹; BADENES, M. L.¹

Source: Molecular Ecology Notes, Volume 6, Number 3, September 2006 , pp. 789-791(3)

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Abstract:

Sixteen simple sequence repeats (SSRs) of apricot (Prunus armeniaca L.) were isolated from a bacterial artificial chromosome (BAC) library. Twelve restriction fragment length polymorphism (RFLP) probes mapped on LG1 of the Prunus general map were hybridized to the BAC library in order to select clones belonging to G1 linkage group of apricot. Selected BACs were digested, subcloned and hybridized with probes containing repeat motifs (GA)₁₀ and (TA)₁₀. Sequencing of the positive subclones revealed 18 unique SSR sequences of which 16 allowed the design of primers flanking the SSR. From the 16 primer pairs, 10 amplified polymorphic markers with an average of observed and expected heterozygosities of 0.44 and 0.68, respectively. The procedure described here proves to be a useful technique for obtaining markers in target areas of a genome.

Keywords: BAC library; Prunus armeniaca; SSR

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1471-8286.2006.01346.x

Affiliations: 1: Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial, 46113 Moncada, Valencia, Spain, 2: Department of Genetics, Biochemistry and Life Science Studies, Clemson University, 100 Jordan Hall, Clemson, SC 29634, USA

Publication date: 2006-09-01