Development and characterization of microsatellite markers for the Amazonian blackwing hatchetfish, Carnegiella marthae (Teleostei, Gasteropelecidae)

Authors: BEHEREGARAY, L. B.¹; PIGGOTT, M.¹; CHAO, N. L.²; CACCONE, A.³

Source: Molecular Ecology Notes, Volume 6, Number 3, September 2006 , pp. 787-788(2)

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Abstract:

The blackwing hatchetfish, Carnegiella marthae, is a small characin species distributed in forest streams of the Negro and upper Orinoco River basins in Amazonia. Freshwater hatchetfish are popular in the aquarium trade and represent an economic resource for the riverine people from middle Rio Negro, in Brazil. We isolated and characterized seven microsatellite DNA loci for the blackwing hatchetfish. Number of alleles and heterozygosity per locus in a sample of 30 fish ranged from three to 17 and from 0.19 to 0.87, respectively. These microsatellite loci provide powerful markers for studies on taxonomy, management and phylogeographic history of Amazonian hatchetfish.

Keywords: Amazon rainforest; Carnegiella marthae; conservation genetics; Gasteropelecidae; microsatellites; phylogeography

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1471-8286.2006.01344.x

Affiliations: 1: Molecular Ecology Laboratory, Department of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia, 2: Departamento de Ciências Pesqueiras, Universidade Federal do Amazonas, Manaus, AM 69700-000, Brazil, 3: Department of Ecology and Evolutionary Biology and Yale Institute of Biospheric Studies, Yale University, New Haven, CT 06520-8106, USA

Publication date: 2006-09-01