Molecular markers for systematic identification and population genetics of the invasive Ponto-Caspian freshwater gammarid Dikerogammarus villosus (Crustacea, Amphipoda)

Authors: WATTIER, R. A.¹; BEGUET, J.¹; GAILLARD, M.¹; MÜLLER, J. C.²; BOLLACHE, L.¹; PERROT-MINNOT, M.-J.¹

Source: Molecular Ecology Notes, Volume 6, Number 2, June 2006 , pp. 487-489(3)

Abstract:

The Ponto-Caspian amphipod, Dikerogammarus villosus, is an invasive species of many European rivers. First, we show that size difference of nrDNA ITS1 allows discriminating D. villosus from Dikerogammarus bispinosus, a closely related but morphologically hardly distinguishable species. Second, we present two types of polymorphic markers for D. villosus, three microsatellites and two single nucleotide polymorphisms (SNPs) of mtDNA COI gene, which were scored by polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP). These markers will be very useful in studying population genetics of D. villosus.

Keywords: COI; invasive species; microsatellite; nrDNA-ITS1; SSCP

Document Type: Research article

DOI: 10.1111/j.1471-8286.2006.01283.x

Affiliations: 1: Equipe Ecologie Evolutive, UMR CNRS 5561 Biogéosciences, Université de Bourgogne, 6 boulevard Gabriel, 21000 Dijon, France, 2: Institute for Medical Statistics and Epidemiology, Ismaninger Str. 22, D-81675, Munich, Germany

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