Novel microsatellite DNA markers for the threatened African endemic tree species, Milicia excelsa (Moraceae), and cross-species amplification in Milicia regia

Authors: OUINSAVI, CHRISTINE; SOKPON, NESTOR¹; BOUSQUET, JEAN²; NEWTON, CRAIG H.³; KHASA, DAMASE P.²

Source: Molecular Ecology Notes, Volume 6, Number 2, June 2006 , pp. 480-483(4)

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Abstract:

Eleven microsatellite primer pairs were developed for the tropical African tree Milicia excelsa. Genomic DNA was enriched for dinucleotide (TC_n and TG_n) and tretranucleotide (GATA_n), and 188 random clones were sequenced from both orientations. We designed and tested 44 oligonucleotide primer pairs, which were evaluated using genomic DNA from 30 M. excelsa mature trees collected from a natural population in Benin. Eleven of the 44 markers showed good amplification and were polymorphic. The number of putative alleles for polymorphic primer pairs varied from three to seven, with expected and observed heterozygosities ranging from 0.10 to 0.64 and from 0.10 to 0.80, respectively. All 11 loci amplified the related species Milicia regia, indicating that these primers will be useful for population and ecology genetic studies in other species of the genus Milicia.

Keywords: African tree species; enriched genomic library; microsatellites; Milicia

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1471-8286.2006.01281.x

Affiliations: 1: Laboratoire d'Études et de Recherches Forestières, Faculté d'Agronomie, Université de Parakou, BP 123 Parakou, République du Bénin, 2: Chaire de Recherche du Canada en Génomique Forestière et Environnementale and Centre de Recherche en Biologie Forestière, Université Laval, Sainte-Foy, Québec, Canada, G1K 7P4, 3: Vizon SciTecInc., 3650 Wesbrook Mall, Vancouver, British Columbia, Canada, V6S 2L2

Publication date: 2006-06-01