Characterization and evaluation of microsatellite loci in European hazelnut (Corylus avellana L.) and their transferability to other Corylus species

Authors: BOCCACCI, P.¹; AKKAK, A.¹; BASSIL, N. V.²; MEHLENBACHER, S. A.³; BOTTA, R.

Source: Molecular Ecology Notes, Volume 5, Number 4, December 2005 , pp. 934-937(4)

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Abstract:

In this work, 18 microsatellite loci were developed in the European hazelnut (Corylus avellana L.) using three enriched genomic libraries. They were evaluated on a set of 20 accessions of this species on the basis of number of alleles (mean: 7.1), expected heterozygosity (mean: 0.67), power of discrimination (mean: 0.77) and polymorphism information content (mean: 0.64). Cross-species transferability was evaluated using seven other Corylus species. All primer pairs amplified in all species, except for CaT-C505 in Corylus ferox and CaT-A114 in Corylus californica.

Keywords: cross-species amplification; filbert; polymorphism; simple sequence repeats

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1471-8286.2005.01121.x

Affiliations: 1: Dipartimento di Colture Arboree, Università di Torino, Via Leonardo da Vinci 44, 10095 Grugliasco (TO), Italy, 2: USDA-ARS National Clonal Germplasm Repository, 33447 Peoria Rd., Corvallis, OR 97331 USA, 3: Department of Horticulture, Oregon State University, 4017 Agricultural and Life Sciences Bldg., Corvallis, OR 97331 USA

Publication date: 2005-12-01