Isolation and characterization of EST-derived microsatellite loci from the fungal wheat pathogen Phaeosphaeria nodorum

Authors: STUKENBROCK, EVA H.; BANKE, SØREN¹; ZALA, MARCELLO¹; MCDONALD, BRUCE A.¹; OLIVER, RICHARD P.²

Source: Molecular Ecology Notes, Volume 5, Number 4, December 2005 , pp. 931-933(3)

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Abstract:

Eleven polymorphic microsatellite loci and one minisatellite locus originating from expressed sequence tag (EST) libraries of Phaeosphaeria (syn. Stagonospora) nodorum were isolated and characterized. The satellite markers were used to genotype isolates from field populations collected in China, North America and South Africa. The number of alleles per locus ranged from two to 15. Genotype diversity ranged from 87.5 to 95.3 and gene diversity from 0.1 to 0.8. The variable levels of polymorphism within and among populations of P. nodorum renders these 12 satellite loci ideal markers for population genetic analysis of P. nodorum.

Keywords: ESTs; leaf blotch; microsatellites; Phaeosphaeria nodorum; population genetics; Stagonospora nodorum

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1471-8286.2005.01120.x

Affiliations: 1: Institute for Plant Sciences, Plant Pathology Group, ETH Zentrum/LFW, Universitätstrasse 2, CH-8092 Zurich, Switzerland, 2: Australian Centre for Necrotrophic Fungal Pathogens, Health Sciences, Murdoch University, Perth, WA 6150, Australia

Publication date: 2005-12-01