Characterization of microsatellite DNA loci for the southern flying squirrel (Glaucomys volans)

Authors: Fokidis H.B.; Schable N.A.¹; Hagen C.¹; Glenn T.C.¹; Risch T.S.²

Source: Molecular Ecology Notes, Volume 3, Number 4, October 2003 , pp. 616-618(3)

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Abstract:

Polymerase chain reaction primers for microsatellite DNA loci (one dinucleotide, four tetranucleotide and two compound) and the conditions necessary to amplify each are described for the southern flying squirrel (Glaucomys volans). These primers were tested on 22 or more individuals from a population at the Savannah River Site in South Carolina. These microsatellite primers yielded a high allelic diversity (6–22 alleles/locus), and moderate to high observed heterozygosities (0.318–0.826). Primers developed for the northern flying squirrel (Glaucomys sabrinus) were also tested for use on G. volans, with only two successful cross amplifications from the seven loci.

Keywords: dinucleotide repeats; Glaucomys volans; microsatellite; polymerase chain reaction; primer; tetranucleotide repeats

Document Type: Research article

DOI: http://dx.doi.org/10.1046/j.1471-8286.2003.00530.x

Affiliations: 1: Savannah River Ecology Laboratory, P.O. Drawer E, Aiken, SC 29802, USA, 2: Department of Biological Sciences, Arkansas State University, PO Box 599, State University, Arkansas, 72467, USA

Publication date: 2003-10-01