Isolation of 39 polymorphic microsatellite loci and the development of a fluorescently labelled marker set for the Eurasian badger (Meles meles) (Carnivora: Mustelidae)

Authors: Carpenter P.J.¹; Dawson D.A.; Greig C.²; Parham A.²; Cheeseman C.L.³; Burke T.

Source: Molecular Ecology Notes, Volume 3, Number 4, October 2003 , pp. 610-615(6)

Publisher: Blackwell Publishing

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Abstract:

We have isolated 78 microsatellite loci from the Eurasian badger (Meles meles). Of the 52 loci characterized, 39 were found to be polymorphic. A fluorescently labelled primer set was developed to enable individual-specific 17-locus genotypes to be obtained efficiently.

Keywords: badger; DNA profile; fluorescent set; Meles; microsatellite; Mustelidae

Document Type: Research article

DOI: 10.1046/j.1471-8286.2003.00529.x

Affiliations: 1: Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK, 2: Department of Biology, University of Leicester, Leicester LE1 7RH, UK, 3: Central Science Laboratory, Sand Hutton, York, YO41 1LZ, UK

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