Development and characterization of 10 novel microsatellite markers from Chital deer (Cervus axis) and their cross–amplification in other related species

Authors: Gaur A.; Singh A.; Arunabala V.; Umapathy G.; Shailaja K.; Singh L.

Source: Molecular Ecology Notes, Volume 3, Number 4, October 2003 , pp. 607-609(3)

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Abstract:

Ten polymorphic microsatellite loci were isolated and characterized from Chital deer (Cervus axis). These loci show high levels of allelic diversity with four to eight alleles per locus in the 22 individuals of the free-ranging population of Chital deer in Nehru Zoological Park, Hyderabad. In addition, we found that all the loci show cross–amplification in closely related as well as distantly related deer species. The amplification of these markers in different genera further indicates that these can be applied to a wide range of endangered deer species for their population genetics studies and conservation management.

Keywords: Cervus axis; Chital deer; cross-species amplification; genetic variation; microsatellite

Document Type: Research article

DOI: http://dx.doi.org/10.1046/j.1471-8286.2003.00528.x

Affiliations: 1: Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India

Publication date: 2003-10-01