Microsatellite markers for Ceiba pentandra (Bombacaceae), an endangered tree species of the Amazon forest

Authors: Brondani R.P.V.¹; Gaiotto F.A.²; Missiaggia A.A.²; Kirst M.³; Gribel R.⁴; Grattapaglia D.

Source: Molecular Ecology Notes, Volume 3, Number 2, June 2003 , pp. 177-179(3)

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Abstract:

From a genomic library enriched for AG/TC repeats, eight polymorphic microsatellite markers were developed for Ceiba pentandra, a pan-tropical forest tree. Polymorphism was evaluated using a panel of 74 adult trees. Using automated fluorescence detection, a total of 112 alleles was detected with an average of 14 alleles per locus. All microsatellite loci showed very high levels of genetic information content, with expected heterozygosity ranging from 0.814 to 0.895. These microsatellite markers represent a powerful tool to investigate refined questions of mating systems, gene flow, family structure and population dynamics in natural populations of C. pentandra.

Keywords: Bombacaceae; Ceiba pentandra; microsatellite; tropical tree

Document Type: Research article

DOI: http://dx.doi.org/10.1046/j.1471-8286.2003.00389.x

Affiliations: 1: Embrapa CNPAF, C.P. 179 Goiânia — GO 74001–970, Brazil, 2: Departamento de Genética — ESALQ/USP — Avenue, Pádua Dias, 11 CP 83 Piracicaba — SP 13400–970, Brazil, 3: Embrapa CENARGEN CP 02372 70770–900 Brasília DF Brazil, 4: Instituto Nacional de Pesquisas da Amazônia – Coordenação de Botânica, CP 478, Manaus — AM 69011–970, Brazil,

Publication date: 2003-06-01