A panel of microsatellites developed for meerkats (Suricata suricatta) by cross-species amplification and species-specific cloning

Authors: Griffin, A. S.¹; Nürnberger, B.²; Pemberton, J. M.¹

Source: Molecular Ecology Notes, Volume 1, Numbers 1-2, March 2001 , pp. 83-85(3)

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Abstract:

We describe the compilation of a panel of 12 polymorphic microsatellite loci suitable for inference of parentage and relatedness in the cooperatively breeding meerkat (Suricata suricatta). First, three of 70 microsatellite primer pairs characterized from other carnivores, specifically cat (Felix catta), dog (Canis familiaris) and grey seal (Halichoerus grypus) revealed polymorphic microsatellites in meerkats. Second, a further nine loci were isolated from meerkats by screening a plasmid library with a ³²P-labelled (CA)₁₅ probe.

Keywords: cross-species amplification; meerkat; microsatellite; species-specific cloning; Suricata suricatta

Document Type: Research article

DOI: http://dx.doi.org/10.1046/j.1471-8278.2001.00035.x

Affiliations: 1: Institute of Cell, Animal and Population Biology, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh EH9 3JT, UK, 2: Zoologisches Institut, Ludwig-Maximilians-Universität, Karlstr. 23, 80333 München, Germany

Publication date: 2001-03-01