Microsatellite loci in Gymnadenia conopsea, the fragrant orchid

Authors: Gustafsson, Susanne; Thorén, Peter A.

Source: Molecular Ecology Notes, Volume 1, Numbers 1-2, March 2001 , pp. 81-82(2)

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Abstract:

Six polymorphic microsatellite loci were found in two genomic libraries (one unenriched and one enriched) constructed using DNA originating from the fragrant orchid, Gymnadenia conopsea. These six microsatellite loci (Gc 17, 29, 42, 49, 51 and 77) showed high levels of variation (H_O= 0.65, 0.90, 0.85, 0.75, 0.90 and 0.40, respectively) when 20 individuals from one population of G. conopsea were PCR amplified and separated in 6% PAA gels.

Keywords: Gymnadenia conopsea; microsatellite; orchid

Document Type: Research article

DOI: http://dx.doi.org/10.1046/j.1471-8278.2001.00033.x

Affiliations: 1: Department of Conservation Biology and Genetics, Uppsala University, Norbyvägen 18D, Box 585, S-752 36 Uppsala, Sweden

Publication date: 2001-03-01