Total bacterial populations in three lentic water bodies of the Colombian Andes using the epifluorescence technique
The epifluorescence technique was used to quantify the total bacterial population in two dams (Neusa and Prado) and in a natural lagoon (Fúquene), which have different geographical locations and physicochemical characteristics. Bacterial abundance was similar in the three water-bodies (8.4 × 108 cells L–1), but the density of plateable heterotrophs was always lower (1.4 × 106 CFU L–1) when obtained by the plate count method. Results obtained during validation of the epifluorescence technique show: (i) that there are no statistically significant differences between counts of samples fixed in the field and unfixed samples; (ii) that the median count of samples fixed and observed periodically over a period of 8 months was similar; (iii) that plate counts underestimate the population; and (iv) that epifluorescence counts were similar in the three water-bodies studied, despite their different ecological conditions.
Document Type: Research Article
Affiliations: Microbiology Laboratory and Scientific Research and Environmental Studies Center, Universidad de Bogotá Jorge Tadeo Lozano, Carrera 4 no. 22–61, Bogotá, Colombia
Publication date: June 1, 2001