Molecular characterization of multiple xylanase producing thermophilic/thermotolerant fungi isolated from composting materials

Authors: Sharma, M.; Chadha, B.S.; Kaur, M.; Ghatora, S.K.; Saini, H.S.

Source: Letters in Applied Microbiology, Volume 46, Number 5, May 2008 , pp. 526-535(10)

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Abstract:

Aims:

Molecular characterization of commercially important group of xylanase producing thermophilic/thermotolerant fungi. Methods and Results:

DNA from 16 thermophilic/thermotolerant fungal isolates was amplified by PCR using three sets of primers: (i) internal transcribed spacer sequence (ITSI-5·8S-ITSII), (ii) D1/D2 hyper variable region of 26S rDNA and (iii) 18S rDNA region. The amplified products of internal transcribed spacers (ITS) and D1/D2 region were sequenced and analysed using ClustalX, whereas, amplified 18S rDNA region was subjected to RFLP analysis based on restriction digestion with RsaI, MboI and Hinf I. Conclusions:

The sequence based analyses of ITSI-5·8S-ITSII as compared with D1/D2 region of 26-28S rDNA was found to be a better tool for phylogenetic resolution of thermophilic/thermotolerant fungi. The ITSI-5·8S-ITSII sequence-based dendrogram indicates an early divergence of the alkaline active xylanase producing thermophilic fungal strains. Significance and Impact of the Study:

This study was the first report on phylogenetic characterization of thermophilic/thermotolerant fungi.

Keywords: alkaline active xylanases; ITS and 26S rDNA sequences; molecular characterization; RFLP of 18S rDNA; thermophilic fungi

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1472-765X.2008.02357.x

Publication date: 2008-05-01