Authors: Qi, C.; Zhu, W.¹; Niu, Y.; Zhang, H. G.²; Zhu, G. Y.³; Meng, Y. H.; Chen, S.; Jin, G.⁴

Source: Journal of Viral Hepatitis, Volume 16, Number 11, November 2009 , pp. 822-832(11)

Publisher: Wiley-Blackwell

Abstract:

Summary. 

A biosensor based on imaging ellipsometry (BIE) has been developed and validated in 169 patients for detecting five markers of hepatitis B virus (HBV) infection. The methodology has been established to pave the way for clinical diagnosis, including ligand screening, determination of the sensitivity, set-up of cut-off values (CoVs) and comparison with other clinical methods. A matrix assay method was established for ligand screening. The CoVs of HBV markers were derived with the help of receiver operating characteristic curves. Enzyme-linked immunosorbent assay (ELISA) was the reference method. Ligands with high bioactivity were selected and sensitivities of 1 ng/mL and 1 IU/mL for hepatitis B surface antigen (HBsAg) and surface antibody (anti-HBs) were obtained respectively. The CoVs of HBsAg, anti-HBs, hepatitis B e antigen, hepatitis B e antibody and core antibody were as follows: 15%, 18%, 15%, 20% and 15%, respectively, which were the percentages over the values of corresponding ligand controls. BIE can simultaneously detect up to five markers within 1 h with results in acceptable agreement with ELISA, and thus shows a potential for diagnosing hepatitis B with high throughput.

Keywords: hepatitis B; imaging ellipsometry; label-free biosensor; microfluidic

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1365-2893.2009.01123.x

Affiliations: 1: Radiation Medical Institute of Shandong Academy of Medical Sciences, Jinan 2: Institute of Microcirculation, Peking Union Medical College & Chinese Academy of Medical Science, Beijing 3: Deparment of Cardiology, Shandong Provincial Hospital, Jinan 4: Institute of Mechanics, Chinese Academy of Sciences, Beijing

Publication date: 2009-11-01

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