X-ray induced reduction of the crystal of high-molecular-weight cytochrome c revealed by microspectrophotometry: X-ray induced reduction of cytochrome c

Authors: Sato M.; Shibata N.; Morimoto Y.; Takayama Y.; Ozawa K.; Akutsu H.; Higuchi Y.; Yasuoka N.

Source: Journal of Synchrotron Radiation, Volume 11, Number 1, 1 January 2004 , pp. 113-116(4)

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Abstract:

The crystal structures of high-molecular-weight cytochrome c (HMC) from Desulfovibrio vulgaris Hildenborough in the transient and reduced states have been determined at 2.8 Å resolution. An absorption spectrum measured with microspectrophotometor indicated that about 86% of the hemes were reduced after 45 min irradiation of the X-ray beam. Further exposure for 90 min did not significantly change the spectrum. These results suggest that HMC in the crystalline state is easily reduced by illumination of the X-ray beam from synchrotron radiation.

Keywords: high-molecular-weight cytochrome c; microspectroscopic measurement; 16 heme proteins; transient state; X-ray crystal structure

Document Type: Research article

DOI: http://dx.doi.org/10.1107/S0909049503023860

Publication date: 2004-01-01