Free Content

Serum proteomic changes in adults with obstructive sleep apnoea

Authors: JURADO‐GAMEZ, BERNABE1; GOMEZ‐CHAPARRO, JOSE LUIS2; MUÑOZ‐CALERO, MARIA3; SERNA SANZ, ANTONIO4; MUÑOZ‐CABRERA, LUIS1; LOPEZ‐BAREA, JUAN2; GOZAL, DAVID5

Source: Journal of Sleep Research, Volume 21, Number 2, 1 April 2012 , pp. 139-146(8)

Publisher: Wiley-Blackwell

Buy & download fulltext article:

You have access to the full text article on a website external to ingentaconnect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library

Abstract:

Summary

To examine whether differentially expressed proteins are present in the serum of patients with obstructive sleep apnoea (OSA), iTRAQ techniques (isobaric tags for relative and absolute quantification) were employed in a prospective study. Individuals were assigned to either a non‐OSA control group (apnoea–hypopnoea index, AHI <5) or an OSA group (AHI ≥5). Blood samples were collected, aliquoted and frozen at −80 °C. Protein digestion and tagging with iTRAQ4plex® and mass spectrometry analysis was then performed (MALDI TOF/TOF). Ten male subjects were included in the control group (age = 45 ± 9.7 years) and 30 male patients in the OSA group (age = 45 ± 10.7 years), the latter being then subdivided into three severity groups. A total of 103 proteins were identified with differential levels between patients with OSA and controls. Of these, 11 proteins were underexpressed and 19 were overexpressed in patients with OSA. C4BPA and thrombospondin were underexpressed in all three OSA severity groups. Among the overexpressed proteins, 13 were overexpressed in the mild OSA group, seven in the moderate group and five in the severe group. Analysis of interactions between the identified proteins revealed that protein alterations in OSA are primarily associated with derangements in lipid and vascular metabolic pathways. This study provides initial evidence that differential protein expression occurs in adults with OSA, and that such proteins change according to disease severity, and appear to primarily involve lipid and vascular metabolic pathways.

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1365-2869.2011.00955.x

Affiliations: 1: Sleep Unit, Department of Respiratory Medicine, Reina Sofia University Hospital, Cordoba, Spain 2: Department of Biochemistry and Molecular Biology, University of Cordoba, Spain 3: Department of Clinic Biochemistry, Reina Sofia University Hospital, Cordoba, Spain 4: Specialist Support MSS Europe, ABSciex, Madrid, Spain 5: Department of Pediatrics, Comer Children’s Hospital, the University of Chicago, USA

Publication date: April 1, 2012

Tools

Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content

Text size:

A | A | A | A
Share this item with others: These icons link to social bookmarking sites where readers can share and discover new web pages. print icon Print this page