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Free Content Heat-shock protein 70: expression in monocytes of patients with sleep apnoea and association with oxidative stress and tumour necrosis factor-α

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Abstract:

Summary

Obstructive sleep apnoea (OSA) is associated with a variety of nightly stresses, including intermittent hypoxaemia, oxidative stress and sleep fragmentation. Heat-shock proteins (HSPs) are upregulated in response to an array of environmental and metabolic stresses. We hypothesized that the OSA-related stresses would affect the expression of HSP70 in monocytes. Basal (30 min, at 37 °C), heat stress-induced HSP70 (30 min, at 43 °C) and basal tumour necrosis factor-α (TNF-α) were determined by flow cytometry in monocytes of 10 patients with OSA and 10 controls matched by age, gender and body mass index. Oxidative stress was determined by thiobarbituric acid-reactive substances (TBARS) and antioxidant paraoxonase-1 activity. Basal HSP70 expression was 1.8-fold higher in patients with OSA as compared with controls (P <0.0005) and was significantly positively correlated with TBARS (r =0.56, P <0.009). However, induction of HSP70 in response to heat stress treatment was lower by 40% in OSA monocytes as compared with controls (P <0.0003). Furthermore, heat stress-induced HSP70 expression was significantly negatively correlated with basal HSP70 expression independently of apnoea severity (r = −0.69, P <0.0006). Also, basal intracellular TNF-α expression was inversely correlated with heat-shock-induced HSP70 (r = −0.78, P <0.015) in OSA monocytes but not in controls. In conclusion, basal HSP70 overexpression that is a protective mechanism indicative of disease-associated stress was significantly higher in patients with OSA and was correlated with oxidative stress. On the other hand, in response to a defined heat-stress treatment, the induction of HSP70 was lower in patients with OSA, indicative of a possible maladaptive response to an acute stress. Correlations with oxidative stress and TNF-α further support this conclusion.

Keywords: heat-shock protein 70; monocytes; oxidative stress; sleep apnoea

Document Type: Research Article

DOI: https://doi.org/10.1111/j.1365-2869.2009.00789.x

Publication date: 2010-03-01

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