Skip to main content

Free Content Increased sodium–proton antiporter activity in patients with obstructive sleep apnoea

Download Article:

You have access to the full text article on a website external to Ingenta Connect.

Please click here to view this article on Wiley Online Library.

You may be required to register and activate access on Wiley Online Library before you can obtain the full text. If you have any queries please visit Wiley Online Library

Abstract:

Cytosolic pH (pHi) and the activity of the sodium–proton antiporter (Na+/H+ antiporter) were measured in lymphocytes from 22 patients with obstructive sleep apnoea and from 24 age-matched healthy subjects (Controls). The cellular Na+/H+ antiporter was measured spectrophotometrically using a pH-sensitive fluorescent dye after intracellular acidification using sodium propionate. Resting pHi was similar in lymphocytes from patients with obstructive sleep apnoea and from controls (7.36 ± 0.20, n=22; vs. 7.35 ± 0.19, n=24; mean ± SD). The Na+/H+ antiporter activity was significantly higher in patients with obstructive sleep apnoea than in controls (11.87 ± 3.26 × 10–3 pHi/s vs. 4.38 ± 1.40 × 10–3 pHi/s; P < 0.0001). The apparent affinity of the Na+/H+ antiporter was not significantly different between the groups (6.90 ± 0.23 vs. 6.87 ± 0.20). In patients with obstructive sleep apnoea the activity of the Na+/H+ antiporter remained stable during the night. The activity of the Na+/H+ antiporter was 13.49 ± 4.80 × 10–3 pHi/s at 20.00 and 13.26 ± 6.13 × 10–3 pHi/s at 02.00. From the present results it is concluded that an increased cellular Na+/H+ antiporter activity may be a genetic marker for patients who are predisposed to obstructive sleep apnoea.

Keywords: fluorescent dye; lymphocytes; obstructive sleep apnoea; sodium–proton antiporter

Document Type: Research Article

DOI: http://dx.doi.org/10.1046/j.1365-2869.2000.00207.x

Affiliations: Medizinische Klinik I, Universitätsklinik Marienhospital, Ruhr-Universität Bochum, Germany

Publication date: September 1, 2000

bsc/jsr/2000/00000009/00000003/art00010
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more