Skip to main content

Rate of metabolic decarboxylation of leucine as assessed by a l[1-13C1]leucine breath test combined with indirect calorimetry of broiler chickens fed isocaloric diets with different protein:fat ratio

Buy Article:

$51.00 plus tax (Refund Policy)

Abstract:

Summary

Research has shown that broiler chickens reared on a low-protein diet have a more efficient protein digestion. However, information on the fate of absorbed amino acids in relation to the dietary crude protein level in poultry is sparse. Therefore, this study aimed at developing a methodology for a 1-13C1-leucine breath test combined with indirect calorimetry, and to apply this technique using broiler diets known to induce differences in protein retention. From 14 days of age onwards, broiler chickens were reared on one of two isocaloric diets with substitutions between fat and protein [low-protein (LP) vs. high-protein (HP) diet: 130.4 vs. 269 g protein/kg; and 101.8 vs. 27.9 g fat/kg]. Every 4 or 5 days, three chickens per diet were placed in the respiratory cells for 48 h. The broilers were intubated with 40 mg 1-13C1-leucine/kg body weight, followed by breath sampling for 4 h at 15-min intervals and mass spectrometric analysis of the 13C:12C ratio in the samples. The CO2 level in the respiratory cell air was monitored and excreta samples were collected. The methodology to studyl[1-13C1]leucine decarboxyation in chickens using a breath test combined with indirect calorimetry was accomplished. Results of the nitrogen balance test indicated that the LP broilers had an improved dietary protein retention compared with the HP animals. Moreover, LP chickens decarboxylated a significantly lower percentage ofl[1-13C1]leucine, demonstrating several ‘protein- or amino acid-sparing’ mechanisms in animals reared on a diet with lower protein level, both at the digestive and at the postabsorptive level.

Keywords: L[1-13C1]leucine; breath test; broiler chickens; dietary protein level

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1439-0396.2006.00661.x

Affiliations: 1:  Laboratory of Livestock Physiology, Immunology and Genetics, Department of Biosystems, K.U.Leuven, Heverlee, Belgium 2:  Laboratory of Animal Nutrition, Ghent University, Merelbeke, Belgium 3:  Department of Gastroenterology, K.U.Leuven, Leuven, Belgium

Publication date: August 1, 2007

bsc/jpn/2007/00000091/F0020007/art00009
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more