Microsatellite Markers Reveal Genetic Variation within Sclerotinia sclerotiorum Populations in Irrigated Dry Bean Crops in Brazil

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Abstract:

Abstract

Microsatellites are powerful markers to infer population genetic parameters. We used 10 microsatellite loci to characterize the genetic diversity and structure of 79 samples of Sclerotinia sclerotiorum isolated from four Brazilian dry bean populations and observed that eight of them were polymorphic within populations. We identified 102 different haplotypes ranging from 6 to 18 per locus. Analyses based on genetic diversity and fixation indices indicated variability among and within populations of 28.79% (FST = 28793) and 71.21%, respectively. To examine genetic relatedness among S. sclerotiorum isolates, we used internal spacer (ITS1-5.8S-ITS2) restriction fragment length polymorphism (PCR-RFLP) and sequencing analysis. PCR-RFLP analysis of these regions failed to show any genetic differences among isolates. However, we detected variability within the sequence, which does not support the hypothesis of clonal populations within each population. High variability within and among populations may indicate the introduction of new genotypes in the areas analysed, in addition to the occurrence of clonal and sexual reproduction in the populations of S. sclerotiorum in the Brazilian Cerrado.

Keywords: DNA fingerprinting; Phaseolus vulgaris L; Sclerotinia sclerotiorum; genetic diversity; multilocus haplotypes; white mould

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1439-0434.2010.01724.x

Affiliations: 1: Authors’ addresses: Instituto de Ciências Biológicas, Universidade Federal de Goiás, 74001-940, Goiânia, Goiás, Brazil 2: EMBRAPA – Centro de Pesquisa Agropecuária dos Cerrados, Caixa Postal 08223, 73301-970, Planaltina, DF, Brazil (correspondence to S. Petrofeza. ), Email: petrofez@uol.com.br

Publication date: February 1, 2011

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