Changes of lipocalin-type prostaglandin D synthase level during pregnancy

Authors: Shiki Y.¹; Shimoya K.; Tokugawa Y.¹; Kimura T.¹; Koyama M.¹; Azuma C.¹; Murata Y.¹; Eguchi N.²; Oda H.³; Urade Y.²

Source: The Journal of Obstetrics and Gynaecology Research, Volume 30, Number 1, February 2004 , pp. 65-70(6)

Publisher: Wiley-Blackwell

Abstract:

Aim:

Prostaglandin D (PGD), synthesized by lipocalin-type prostaglandin D synthase (L-PGDS), has marked effects on a number of biological processes, including the prevention of platelet aggregation and the relaxation of vascular smooth muscle. The aim of the study presented here was to examine the significance of L-PGDS in human pregnancy. Methods:

We measured the concentration of plasma L-PGDS in pregnant and non-pregnant women, and the concentration of L-PGDS in the umbilical cord blood, amniotic fluid and urine of newborns by enzyme-linked immunoabsorbent assay. To determine the localization of L-PGDS, we performed immunohistochemical analysis. To evaluate the usefulness of diagnosis of rupture of membranes (ROM), we determined the concentration of L-PGDS in cervicovaginal secretions. Results:

Pregnant women and non-pregnant women had similar L-PGDS concentrations (0.57 ± 0.13 µg/mL vs 0.53 ± 0.07 µg/mL). Umbilical cord blood, amniotic fluid and newborn urine contained higher L-PGDS concentrations (1.87 ± 0.73 µg/mL, 2.62 ± 0.86 µg/mL, 6.31 ± 4.62 µg/mL, respectively) than maternal blood. The concentration of L-PGDS in amniotic fluid from 19 weeks onward was significantly greater than that at 15–18 weeks (3.201 ± 0.384 µg/mL, n = 6 vs 1.735 ± 0.477 µg/mL, n = 4; P < 0.05). Immunohistochemistry revealed that the amniotic cells of the placenta expressed L-PGDS. The sources of L-PGDS in amniotic fluid are fetus urine and amniotic cells. The concentration of L-PGDS in cervicovaginal secretions with rupture of membrane (ROM) were significantly higher than those without ROM. Conclusion:

The measurement of L-PGDS in cervicovaginal fluid was useful in the detection of ROM during pregnancy.

Keywords: amniotic fluid; chorion; fetal circulation; lipocalin-type prostaglandin D synthase; prostaglandin D

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1341-8076.2004.00158.x

Affiliations: 1: Department of Obstetrics and Gynecology, Osaka University Graduate School of Medicine, 2: Department of Molecular Behavior Biology, Osaka Bioscience Institute, Osaka and 3: Central Research Institute of Maruha, lbaraki, Japan

Publication date: 2004-02-01

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