Terminal Nerve Gonadotrophin-Releasing Hormone (GnRH) Neurones Express Multiple GnRH Receptors in a Teleost, the Dwarf Gourami (Colisa lalia)

Authors: Hajdú, P.; Ikemoto, T.; Akazome, Y.; Park, M. K.; Oka, Y.

Source: Journal of Neuroendocrinology, Volume 19, Number 6, June 2007 , pp. 475-479(5)

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Abstract:

Gonadotophin-releasing hormone (GnRH) peptide released from the terminal nerve (TN)-GnRH neurones of the dwarf gourami primarily modifies the electrical properties of various neurones, including the TN-GnRH neurones themselves. However, our knowledge on the expression of GnRH receptors (GnRHRs) in the TN-GnRH neurones is still limited. Here, we used the single-cell reverse transcriptase-polymerase chain reaction after whole-cell patch-clamp recording to study the distribution of various GnRHR types expressed in the individual TN-GnRH neurones. We found that TN-GnRH neurones express two of the three types of GnRHRs cloned in the dwarf gourami: GnRHR1-2 and -R2, but not -R1-1. Furthermore, in agreement with our previous findings, all TN-GnRH neurones contained mRNAs of salmon GnRH but not chicken GnRH-II.

Keywords: teleost; GnRH; GnRH receptor; terminal nerve GnRH neurones; single-cell RT-PCR

Document Type: Research article

DOI: http://dx.doi.org/10.1111/j.1365-2826.2007.01553.x

Affiliations: 1: Laboratory of Biological Signalling, Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.

Publication date: 2007-06-01