Leptin Receptor Immunoreactivity is Associated with the Golgi Apparatus of Hypothalamic Neurones and Glial Cells
Authors: Diano, Sabrina; Kalra, Satya P.; Horvath, Tamas L.
Source: Journal of Neuroendocrinology, Volume 10, Number 9, September 1998 , pp. 647-650(4)
Abstract:Leptin has emerged as a major peripheral hormone, controlling central mechanisms of metabolism and related autonomic and endocrine functions. In the regulation of hypothalamic neurones, leptin is suggested to affect different second messenger systems and transcription regulating factors. The present study reports the predominant localization of leptin receptor immunoreactivity in the cis and trans cisternae of the Golgi apparatus in hypothalamic neuronal and glial cells. In these hypothalamic cells, translocation of leptin receptor immunoreactivity from the Golgi apparatus to the perikaryal membrane, nucleus or to the cytoplasm was not apparent after manipulation of the metabolic state either by fasting or suppression of the thyroid axis. On the other hand, leptin receptor immunoreactivity was associated with the perikaryal membrane of neurones in other parts of the central nervous system, including the dentate gyrus and the cingulate cortex. These data indicate an extremely high turnover of leptin receptors in hypothalamic target sites, but also raise the possibility that leptin may interact with the Golgi apparatus-related mechanisms to alter intracellular mechanisms.
Document Type: Original Article
Affiliations: Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT and *Department of Neuroscience, University of Florida College of Medicine, Gainesville, FL, USA
Publication date: September 1, 1998