An optical differential scanning calorimeter cryomicroscope
Authors: YUAN, S.1; DILLER, K. R.
Source: Journal of Microscopy, Volume 218, Number 2, May 2005 , pp. 85-93(9)
Publisher: Blackwell Publishing
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Abstract:
Summary An optical-DSC system was designed, built, tested, calibrated and verified to incorporate into a single device the capability for simultaneous optical cryomicroscopy and differential scanning calorimetry (DSC). This instrument can be used to obtain both visual and thermal data for an individual specimen subjected to a defined freezing and thawing protocol with very little compromise in quality or range of data available in comparison with dedicated single instruments. Temperature and caloric calibrations were performed based on phase transition states in water, n-dodecane and n-decane. The instrument has proven effective for process analysis in living cells and in foodstuffs.Keywords: Cryomicroscope; differential scanning calorimeter
Document Type: Short communication
DOI: 10.1111/j.1365-2818.2005.01474.x
Affiliations: 1: Department of Biomedical Engineering, The University of Texas at Austin, 1 University Station, C0800, Austin, TX 78712-1084, U.S.A.
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