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ORGANIC ACIDS PROFILE IN TOMATO JUICE BY HPLC WITH UV DETECTION

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ABSTRACT

A simple method was developed to determine 10 organic acids simultaneously in tomato products using reversed-phase high-performance liquid chromatography column with the diode array detector set at 210 nm. After centrifugation and filtration, the samples were passed through an anion exchange resin and the organic acids were released using 0.1-N HCl. The chromatographic separation was achieved with isocratic analysis in a 20-min run. The method was reliable and sensitive, the coefficient of determination of the standard calibration curve is 0.9925 ≤ r2 ≤ 0.9999 and the limit of detection ranged from 0.08 to 6.00 mg/kg for trans-aconitic acid and acetic acid, respectively. The limit of quantification ranged from 0.19 to 15.18 mg/kg for trans-aconitic and acetic acid, respectively. To establish the efficiency of the anion resin, the procedure was applied to a standard solution of a mixture of organic acids. The organic acids recovery ranged from 87.0% ± 1.9 for citramalic acid to 109.9% ± 5.2 for fumaric acid.
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Document Type: Research Article

Publication date: 2007-04-01

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