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Response surface methodology was used to optimize some operating parameters on the effects of alcalase treatment extraction of lutein, zeaxanthin andβ-cryptoxanthin from corn gluten meal (CGM) on the yield of total xanthophylls. Three independent variables tested were enzyme concentration, substrate concentration and hydrolyzing time. The t-test and P value indicated that the quadratic of the three variables, enzyme concentration and hydrolyzing time were important linear variables affecting the total xanthophyll yield (P < 0.005), followed by substrate concentration (P < 0.01). The interaction effect between enzyme concentration and hydrolyzing time was also significant (P < 0.005). Considering the efficiency, the economy of materials and the feasibility of experiment, the technique parameters were optimized by constrained complex method. The optimum conditions were obtained as follows: the enzyme concentration, the substrate concentration and the hydrolyzing time were 4,367 U/g, 11.3% and 2.4 h, respectively. The highest total xanthophyll yield was predicted of 65.69 µg/g when CGM was hydrolyzed under the optimum condition. The suitability of the model equation for predicting the optimal response values was tested using the selected optimum conditions; the experimental yield was 65.06 ± 0.78 µg/g, which was found to be in agreement with the predicted yield. PRACTICAL APPLICATIONS

Corn gluten meal (CGM), a major by-product of corn wet milling, contains (on a dry basis) 200–400 µg/g carotenoids. There are ∼840,000 tons of CGM produced in China every year. If a part of CGM produced was manufactured further to produce carotenoids as foods, materials of foods and additives of pharmaceutical products, its value and presence in the marketplace would be increased.
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Document Type: Research Article

Publication date: 01 April 2009

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