Autosomal Short Tandem Repeat Analysis of Ancient DNA by Coupled Use of Mini‐ and Conventional STR Kits*
Abstract:Abstract: Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpFlSTR® MiniFiler™ kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n = 8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpFlSTR® MiniFiler™ and Identifiler™ kits. Whereas only 21.87% of larger‐sized loci profiles could be obtained with the Identifiler™ kit, 75% of the same loci profiles were determined by MiniFiler™ kit analysis. This very successful amplification of large‐sized STR markers from highly degraded aDNA suggests that the MiniFiler™ kit could be a useful complement to conventional STR kit analysis of ancient samples.
Document Type: Research Article
Affiliations: 1: Hangang Institute of Cultural Heritage, 294-6, Jungwha 1(il)-Dong, Jungnang-Gu, Seoul 131-877, South Korea 2: Department of Forensic Medicine, Seoul National University College of Medicine, 28, Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea 3: Department of Medicine, University of Wisconsin-Madison, School of Medicine and Public Health, 750 Highland Avenue, Madison, WI 53705 4: Department of Anatomy, Ewha Womans University School of Medicine, 911-1, Mok-6-Dong, Yangcheon-Gu, Seoul 158-710, South Korea.
Publication date: May 1, 2012