If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Comparison of Mitotyper Rules and Phylogenetic-based mtDNA Nomenclature Systems

$48.00 plus tax (Refund Policy)

Download / Buy Article:

Abstract:

Abstract: 

A consistent nomenclature scheme is necessary to characterize a forensic mitochondrial DNA (mtDNA) haplotype. A standard nomenclature, called the Mitotyper Rules™, has been developed that applies typing rules in a hierarchical manner reflecting the forensic practitioner’s nomenclature preferences. In this work, an empirical comparison between the revised hierarchical nomenclature rules and the phylogenetic approach to mtDNA type description has been conducted on 5173 samples from the phylogenetically typed European Mitochondrial DNA Population database (EMPOP) to identify the degree and significance of any differences. The comparison of the original EMPOP types and the results of retyping these sequences using the Mitotyper Rules demonstrates a high degree of concordance between the two alignment schemes. Differences in types resulted mainly because the Mitotyper Rules selected an alignment with the fewest number of differences compared with the rCRS. In addition, several identical regions were described in more than one way in the EMPOP dataset, demonstrating a limitation of a solely phylogenetic approach in that it may not consistently type nonhaplogroup-specific sites. Using a rule-based approach, commonly occurring as well as private variants are subjected to the same rules for naming, which is particularly advantageous when typing partial sequence data.

Keywords: EMPOP; SWGDAM; forensic science; hierarchical bifurcating approach; mitochondrial DNA; nomenclature; phylogenetics

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1556-4029.2010.01477.x

Affiliations: 1: FBI Laboratory, 2501 Investigation Parkway, Quantico, VA 22135. 2: Mitotech LLC, 590 Monte Alto, Santa Fe, NM 87501. 3: University of North Texas, HSC, 3500 Camp Bowie Blvd., Fort Worth, TX 76107.

Publication date: September 1, 2010

Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more