Skip to main content

Validation of Half-Reaction Amplification Using Promega PowerPlex® 16

Buy Article:

$51.00 plus tax (Refund Policy)



DNA amplification is a fundamental yet costly process used in DNA analysis. This study evaluated half-reaction amplification (12.5, 12, and 13 uL) using the Promega Powerplex® 16 Kit with the hope of reducing sample analysis costs by half. A sensitivity study was completed, along with the testing of various blood stain samples including those with low (<0.40 ng) and high DNA concentrations (>3.0 ng), peak height imbalances, and allelic drop-out. Also, 467 samples submitted to the MUFSC laboratory for testing were analyzed. Results indicate that half-reaction amplification produced higher quality profiles than full-reactions. Average peak heights increased by 85%, peak height imbalances improved, and drop-out was eliminated in 75.8% of samples. Only eight of 467 case samples required re-amplification, a success rate of 94% was observed, and the repeat rate decreased significantly. Finally, a DNA input of 0.25–1.0 ng is ideal for half-reaction amplification.

Keywords: CSF; D13S317; D16S539; D18S51; D21S11; D3S51358; D5S818; D7S820; D8S1179; DNA amplification; FGA; Penta D; Penta E; PowerPlex® 16; THO1; TPOX; forensic DNA; forensic science; vWA; validation

Document Type: Research Article


Affiliations: Marshall University Forensic Science Center, DNA Laboratory, 1401 Forensic Science Drive, Huntington, WV.

Publication date: July 1, 2010


Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more