Skip to main content

Age Estimation by Racemization Method in Teeth: Application of Aspartic Acid, Glutamate, and Alanine

Buy Article:

$51.00 plus tax (Refund Policy)



Here, we report on an experimental approach of simultaneous determination of various amino acids racemization (AAR) rates in teeth. We evaluated the measurements of aspartic acid (Asp), glutamate (Glu), and alanine (Ala) isolated from dentin. Asx D/L rates from total amino acid fraction, generally used for age estimation, showed high correlation (r = 0.98) with age. As Glx and Ala showed very slow racemization kinetics in TA, we performed further analysis of the acid-soluble protein (SP) fraction. The results supported improved correlation between age and D/L rates for Glu (r = 0.84) and Ala (r = 0.85), as well as for Asp (r = 0.98). By providing further elucidation on dentin protein racemization, the technique offers a considerable opportunity to involve other amino acids in age estimation studies. As the process does not require additional separation steps, the method can be easily adapted to existing protocols.

Keywords: age estimation; amino acid; dentin; forensic science; odontology; racemization

Document Type: Research Article


Affiliations: 1: Department of Biochemistry, Akita University School of Medicine, Akita, Japan. 2: Institute for Frontier Oral Science and Department of Forensic Medicine, Kanagawa Dental College, Yokosuka, Japan.

Publication date: 2010-05-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more