Development of a Nomenclature System for a Canine STR Multiplex Reagent Kit

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Abstract:

Abstract: 

Despite the popularity of dogs in US households, canine DNA evidence remains largely untapped in forensic investigations partially because of the absence of well-defined forensic short tandem repeats (STRs), lack of standardized and validated PCR protocols, STR reagent kits, and poorly developed nomenclature. A nomenclature system was established based on internationally recognized recommendations for human forensic STRs for a recently developed canine STR reagent kit. Representative alleles were sequenced from each of the 18 STRs and the sex-typing marker included in the kit. This study also reflects on the impact of point mutations, insertions, and deletions within and outside the STR core repeat structures. An understanding of the STRs’ sequence and repeat structures will enable development of a robust and reliable allele nomenclature and improve the accuracy and precision of allele fragment sizing in canine forensic profiling. The expected allele sizes have been calculated, and their repeat stuctures defined based on sequence information.

Keywords: complex repeats; compound repeats; domestic dog; forensic science; microsatellites; nonhuman forensic DNA; simple repeats

Document Type: Research Article

DOI: http://dx.doi.org/10.1111/j.1556-4029.2010.01361.x

Affiliations: 1: Scientific Investigation Division, Los Angeles Police Department, Los Angeles, CA 90012. 2: Finnzymes Diagnostics, Keilaranta 16 A, 02150 Espoo, Finland. 3: The Jan Bashinski DNA Laboratory, California Department of Justice, Richmond, CA 94804. 4: MMI Genomics, Inc., 1756 Picasso Avenue, Davis, CA 95618. 5: Graduate Group in Forensic Science, University of California–Davis, Davis, CA 95616. 6: Department of Anthropology, University of California–Davis, Davis, CA 95616.

Publication date: May 1, 2010

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