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Validation of the AMPFℓSTR® MiniFilerTM PCR Amplification Kit for Use in Forensic Casework

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The AmpFSTR® MiniFilerTM PCR Amplification Kit is designed to genotype degraded and/or inhibited DNA samples when the AmpFSTR® IdentifilerTM PCR Amplification Kit is incapable of generating a complete genetic profile. Validation experiments, following the SWGDAM guidelines, were designed to evaluate the performance of MiniFiler. Data obtained demonstrated that MiniFiler, when used in conjunction with Identifiler, provided an increased ability to obtain genetic profiles from challenged samples. The optimum template range was found to be between 0.2 and 0.6 ng, with 0.3 ng yielding the best results. Full concordance was achieved between the MiniFiler kit and Identifiler kit except in a single case of a null allele at locus D21S11. Numerous instances of severe heterozygous peak imbalance (<50%) were observed in single source samples amplified within the optimum range of input DNA suggesting that caution be taken when attempting to deduce component genotypes in a mixture.
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Keywords: AmpFℓSTR; CSF1PO; D13S317; D16S539; D18S51; D21S11; D2S1338; D7S820; DNA typing; FGA; forensic science; miniSTR; polymerase chain reaction; validation

Document Type: Research Article

Affiliations: 1: Sam Houston State University, Chemistry and Forensic Science Bldg., 1003 Bowers Blvd., Huntsville, TX 77340. 2: San Diego Police Department, 1401 Broadway, San Diego, CA 92101.

Publication date: 2009-09-01

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