Skip to main content

Polymerase Resistance to Polymerase Chain Reaction Inhibitors in Bone

Buy Article:

$43.00 plus tax (Refund Policy)


Amplification of DNA from aged or degraded skeletal remains can be a challenging task, in part due to naturally occurring inhibitors of the polymerase chain reaction. PCR inhibitors may act by inactivating a polymerase itself, or compete with or bind other reaction components, although various polymerases may be differentially susceptible to such insult. In this study, ten thermostable polymerases from six bacterial species were examined for their ability to amplify DNA in the presence of bone-derived or individual PCR inhibitors. Two polymerases, one from Thermus aquaticus and one from Thermus thermophilus, showed lower susceptibility to inhibition from bone, while polymerases from Thermus flavus were highly susceptible. Addition of bovine serum albumin improved the activity of most of the enzymes. Taken together, the results indicate that thermostable DNA polymerases have different susceptibility to bone-derived PCR inhibitors, and that those most often used in forensic laboratories may not be optimal when working with DNA from skeletal remains.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Pyrococcus woesei; Thermococcus gorgonarius; Thermococcus zilligii; Thermus aquaticus; Thermus filiformis; Thermus flavus; Thermus litoralis; Thermus thermophilus; Thermus ubiquitous; bovine serum albumin; forensic science; inhibition; inhibitors; polymerase; polymerase chain reaction

Document Type: Research Article

Affiliations: Forensic Science Program, School of Criminal Justice and Department of Zoology, Michigan State University, East Lansing, MI 48824.

Publication date: 2009-09-01

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more