Skip to main content

Identification of Prostate-Specific Antigen and Spermatozoa from a Mixture of Semen and Simulated Gastric Juice

Buy Article:

$43.00 plus tax (Refund Policy)


The detection of prostate-specific antigen (PSA) and visualization of spermatozoa from forensic-type samples containing semen exposed to simulated gastric juice was investigated as a support for forensic practice. Samples of simulated gastric juice mixed with semen were prepared and incubated for up to 4 h at 37°C. Samples were deposited on cotton cloth and on ceramic plates and allowed to dry. The samples were examined for the presence of PSA using the Seratec® PSA Semiquant immunochromatographic membrane test. Microscope slides were prepared, stained, and analyzed for spermatozoa. Spermatozoa were detected in all samples, and PSA was detected on neat samples and on samples from the ceramic plate after incubation for up to 4 h. PSA was not detected in the samples deposited on cotton cloth at incubation times greater than 15 min. This may serve as a support for examinations performed when vomit or vomit-stained evidence is submitted for analysis.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: drug-facilitated sexual assault; forensic science; prostate-specific antigen; simulated gastric juice; spermatozoa; vomit

Document Type: Research Article

Affiliations: Wyoming State Crime Laboratory, 316 West 22nd, Cheyenne, WY 82002.

Publication date: 01 May 2009

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more