IngentaConnect Sequence Analysis of a New Short Tandem Repeat Locus D17S2266E Lo...

Authors: Spolnicka, Magdalena¹; Jagiello, Agata²; Soltyszewski, Ireneusz¹; Jacewicz, Renata³; Plucienniczak, Andrzej²; Berent, Jaroslaw³

Source: Journal of Forensic Sciences, Volume 53, Number 3, May 2008 , pp. 683-686(4)

Publisher: Blackwell Publishing

Abstract:

D17S2266E is a new, variable genetic marker exhibiting polymorphism of the number of repeats of four- and two-nucleotide motifs. This study, carried out on a group of 250 unrelated persons from various regions of Poland, revealed the presence of 24 different alleles ranging in size from 232 to 290 base pairs. Analysis of the sequenced fragments demonstrated that the alleles consisted of two flanking regions and two variable blocks that were separated by a consensus sequence. There were (AAAG)₅(AG)₁(AAAG)_3-4 repeats in the first block, and [(AAAG)₂(AG)₁]_0-1[(AAAG)₆(AG)₁]_0-1(AAAG)_n repeats in the second block. On the basis of the allele frequencies in the population, we were able to do biostatistical calculations, which gave the following results: expected heterozygosity 0.8947 ± 0.0137, power of discrimination 0.9793, polymorphism information content 0.8837, probability of exclusion (PE) 0.7859, PE for motherless cases 0.6473, and an average paternity index of 4.7470. These biostatistical parameters show that the marker D17S2266E can find a wide range of applications in forensic testing.

Keywords: forensic science; DNA typing; DNA polymorphism; short tandem repeat marker; D17S2266E; HumGH@

Document Type: Research article

DOI: 10.1111/j.1556-4029.2008.00720.x

Affiliations: 1: Department of Biology, Central Forensic Laboratory, General Headquarters of Police, Iwicka 14, 00-735 Warsaw, Poland. 2: Institute of Biotechnology and Antibiotics, Staroscinska 5, 02-516 Warsaw, Poland. 3: Department of Forensic Medicine, Medical University of Lodz, Sedziowska 18a, 91-304 Lodz, Poland.

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$50.39 plus tax Refund Policy

Sequence Analysis of a New Short Tandem Repeat Locus D17S2266E Located in the Human Growth Hormone Gene Cluster HumGH@